Project leader: Dr. S. Bertrand
The molecular epidemiology program has emerged in the bacteriology division from the integration of molecular biology into traditional epidemiologic research.
The objectives of molecular epidemiology are quite broad and include:
1) The performance of molecular analysis of Salmonella, Shigella, Neisseria meningitidis and Listeria outbreaks in order to complete the epidemiologic surveillance of these three National Reference Centers. It consists of:
- molecular analysis of strains that can not be characterized via traditional tools
- genetic characterization of antibiotics resistance mechanisms of a strain presenting e.g resistance against fluoroquinolone or cephalosporin of third generation.
2) The investigation with the pulse field electrophoresis (PFGE) techniques to find the origin of an outbreak.
4) Research projects in order to develop new tools for the epidemiologic surveillance
- Detection of Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumonia using PCR and RFLP
- Characterization of the Neisseria meningitidis serogroup by PCR
- Characterization of Neisseria meningitidis by MultiLocus Sequence Typing (MLST), (MLDF or RFLP) and penA typing
- Investigation of Neisseria meningitidis, Salmonella, Shigella and Listeria outbreaks via pulse field elefectrophoresis
- Detection of Salmonella and Listeria using specific PCR
- Identification of Listeria monocytogenes using specific PCR
- Identification of Salmonella phase 1 and 2 antigens using multiplex PCR
- Identification of Shigella serotypes by RFLP
- Molecular characterization of β –lactamases using specific PCR and sequencing
- Characterization of the quinolone resistance-determining regions (QRDRs) of the gyrA, gyrB parc and par E gene of gram + and gram- strains using PCR and sequencing
- Bacterial identification using biochemical (API, Biolog) and molecular tools (16S ribosomal gene sequencing)